]. Participants completed a 5 min warm-up at a speed corresponding to LT1. Exercise intensity then increased to speeds that corresponded with 60% of the difference between LT1 and LT2 for 50 min, before completing a maximum of 5 × 1 min intervals at a speed 10% above LT2, interspersed with 1 min active recovery at 60% ∆LT1-LT2. B[La], HR and RPE were measured at rest, and at 10, 30 and 48 min, and following the last interval. Exercise intensity was consistent between visit 2 and 4.
Plasma HT was assessed using a liquid–liquid extraction method following acidic hydrolysis, with gas chromatography–mass spectrometry analysis .Nutritional supplementation was distributed in a double-blinded manner to participants upon completion of visit 3. Product dose and timeframe were based on commercial product supply and company recommendations to consume 1 box of OliP as an acute intervention period.